Serological, chemical, and structural analyses of the Escherichia coli cross-reactive capsular polysaccharides K13, K20, and K23
نویسندگان
چکیده
منابع مشابه
Recombinant expression of Streptococcus pneumoniae capsular polysaccharides in Escherichia coli
Currently, Streptococcus pneumoniae is responsible for over 14 million cases of pneumonia worldwide annually, and over 1 million deaths, the majority of them children. The major determinant for pathogenesis is a polysaccharide capsule that is variable and is used to distinguish strains based on their serotype. The capsule forms the basis of the pneumococcal polysaccharide vaccine (PPV23) that c...
متن کاملSerological and genetic diversity of capsular polysaccharides in Enterococcus faecalis.
Enterococci possess capsular carbohydrate antigens that are targets of opsonic antibodies. These antigens may be used to develop alternative options for the treatment and prevention of enterococcal infections. The present study was done to analyze the diversity of capsular polysaccharides in Enterococcus faecalis. Four type-specific sera were used in an enzyme-linked immunosorbent assay format ...
متن کاملEffects of streptomycin and kanamycin on the production of capsular polysaccharides in Escherichia coli B23 cells
Aminoglycosides are a class of antibiotics that inhibit protein synthesis in bacteria by binding to the 30S ribosomal subunit. However, it has been reported that certain aminoglycosides have secondary effects on the cell’s overall carbohydrate concentration. Previous studies have also reported an increase in capsule size. This study attempted to investigate the effects of kanamycin and streptom...
متن کاملPolyacrylamide gel electrophoresis of the capsular polysaccharides of Escherichia coli K1 and other bacteria.
Methods were developed for the polyacrylamide gel electrophoretic analysis of capsular polysaccharides of bacteria with Escherichia coli K1 as a model. Conditions were determined for the rapid and gentle extraction of the K1 polysaccharide by incubation of the bacteria in a volatile buffer and for the subsequent removal of the putative phospholipid moiety attached to the reducing end of the pol...
متن کاملEffects of meningococcal and Escherichia coli capsular polysaccharides on human and dog platelet aggregation in vitro.
Highly purified capsular polysaccharides from groups A, B, and C meningococci and from two strains of Escherichia coli did not aggregate human or dog platelets in vitro. Nor was there any detectable effect on platelet aggregation induced by adenosine diphosphate (ADP), epinephrine, or collagen. The results do not support the hypothesis that capsular polysaccharides are involved in the pathogene...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1983
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.39.2.623-629.1983